Material-driven Fibronectin Assembly Promotes Maintenance of Mesenchymal Stem Cell Phenotypes

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mMSCs under basal conditions. Immunofluorescence for osteogenic, adipogenic, and stemness markers expressed under basal conditions and quantification of staining intensity. Full details in paper.

A new paper was published last month online on the journal Advanced Functional Materials by Patricia Rico, Hayk Mnatsakanyan, Matthew J Dalby and Manuel Salmerón-Sánchez.

The paper, “Material-driven Fibronectin Assembly Promotes Maintenance of Mesenchymal Stem Cell Phenotypes“, describes a material system capable of sustaining the growth of stem cells (maintaining stemness) and of promoting highly efficient differentiation with adequate stimulation.

The control of stem cell fate is a critical tool in the development of regenerative medicine strategies and in the study of fundamental biology mechanisms.

Poly(ethyl acrylate) induces assembly of fibronectin (FN) into nanonetworks (FN fibrillogenesis) upon simple adsorption from solutions. In this paper it is shown that these FN nanonetworks allow growth of MSCs and maintenance of stemness for long periods of time (up to 30 days) using basal media in absence of soluble factors. The system promotes enhanced levels of differentiation when defined supplemented media are used.